amc hn 8 cells  (MedChemExpress)

Journal: Journal of Oncology

Article Title: Overexpression of Laminin 5 γ 2 Chain Correlates with Tumor Cell Proliferation, Invasion, and Poor Prognosis in Laryngeal Squamous Cell Carcinoma

doi: 10.1155/2022/7248064

Figure Lengend Snippet: LAMC2 was highly expressed in LSCC cells. (a) and (b): LAMC2 mRNA and protein expression in LSCC cells texted by qRT-PCR and western blot. (c) and (d): qRT-PCR and western blot were performed to detect the effect of LAMC2 overexpression on LAMC2 mRNA and protein expression. (e) and (f): LAMC2 knockdown inhibited the expression of LAMC2 mRNA and protein in AMC-HN-8 cells. qRT-PCR and Western blot were performed to detected the effect of LAMC2 knockdown on LAMC2 mRNA and protein expression. Data were expressed as mean ± SD, n = 3. Compared to the 16HBE group, ▲▲ P < 0.01. Compared to the vector group, ## P < 0.01. Compared to the shNC group, ∗∗ P < 0.01.

Article Snippet: LSCC cells lines (TU177, AMC-HN-8, TU686, and TU212) were purchased from Cyberkang (Shanghai) Biotechnology Co., Ltd. Human bronchial epithelial cells (16HBE) cells were obtained from ATCC (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Over Expression, Knockdown, Plasmid Preparation

Journal: Journal of Oncology

Article Title: Overexpression of Laminin 5 γ 2 Chain Correlates with Tumor Cell Proliferation, Invasion, and Poor Prognosis in Laryngeal Squamous Cell Carcinoma

doi: 10.1155/2022/7248064

Figure Lengend Snippet: LAMC2 promoted the proliferation and cell cycle of LSCC cells. (a) LAMC2 increased AMC-HN-8 and TU177 cell viability. CCK8 assay was performed to detect the effect of LAMC2 overexpression and knockdown on LSCC cell viability. (b) LAMC2 facilitated the colony formation of AMC-HN-8 and TU177 cells. Colony formation assay was performed to detect the effect of LAMC2 overexpression and knockdown on LSCC cell proliferation. (c) LAMC2 reduced G0/G1 phase in AMC-HN-8 and TU177 cells. Flow cytometry was performed to detect the effect of LAMC2 overexpression and knockdown on LSCC cell cycle. Data were expressed as mean ± SD, n = 3. Compared to the vector group, ## P < 0.01. Compared to the shNC group, ∗∗ P < 0.01.

Article Snippet: LSCC cells lines (TU177, AMC-HN-8, TU686, and TU212) were purchased from Cyberkang (Shanghai) Biotechnology Co., Ltd. Human bronchial epithelial cells (16HBE) cells were obtained from ATCC (Manassas, VA, USA).

Techniques: CCK-8 Assay, Over Expression, Knockdown, Colony Assay, Flow Cytometry, Plasmid Preparation

Journal: Journal of Oncology

Article Title: Overexpression of Laminin 5 γ 2 Chain Correlates with Tumor Cell Proliferation, Invasion, and Poor Prognosis in Laryngeal Squamous Cell Carcinoma

doi: 10.1155/2022/7248064

Figure Lengend Snippet: LAMC2 promoted TU177 and AMC-HN-8 cells migration and invasion. (a)–(b): LAMC2 promoted TU177 and AMC-HN-8 cells migration. Wound healing and Transwell assays were performed to detect the effect of LAMC2 overexpression and knockdown on LSCC cell migration. (c) LAMC2 promoted TU177 and AMC-HN-8 cells invasion. Transwell assay was performed to detect the effect of LAMC2 overexpression and knockdown on LSCC cell invasion. Data were expressed as mean ± SD, n = 3. Compared to the vector group, ## P < 0.01. Compared to the shNC group, ∗∗ P < 0.01.

Article Snippet: LSCC cells lines (TU177, AMC-HN-8, TU686, and TU212) were purchased from Cyberkang (Shanghai) Biotechnology Co., Ltd. Human bronchial epithelial cells (16HBE) cells were obtained from ATCC (Manassas, VA, USA).

Techniques: Migration, Over Expression, Knockdown, Transwell Assay, Plasmid Preparation

Journal: Journal of Oncology

Article Title: Overexpression of Laminin 5 γ 2 Chain Correlates with Tumor Cell Proliferation, Invasion, and Poor Prognosis in Laryngeal Squamous Cell Carcinoma

doi: 10.1155/2022/7248064

Figure Lengend Snippet: LAMC2 promoted the EMT in LSCC cells. (a) Overexpression of LAMC2 promoted the expression of N-cadherin and vimentin proteins in TU177 cells and inhibited the expression of E-cadherin protein. Western blot was performed to detect the effect of LAMC2 overexpression on the EMT in LSCC cells. (b) LAMC2 knockdown inhibited N-cadherin and vimentin protein expression and promoted E-cadherin protein expression in AMC-HN-8 cells. Western blot was performed to detect the effect of LAMC2 knockdown on the EMT in LSCC cells. Data were expressed as mean ± SD, n = 3. Compared to the vector group, ## P < 0.01. Compared to the shNC group, ∗∗ P < 0.01.

Article Snippet: LSCC cells lines (TU177, AMC-HN-8, TU686, and TU212) were purchased from Cyberkang (Shanghai) Biotechnology Co., Ltd. Human bronchial epithelial cells (16HBE) cells were obtained from ATCC (Manassas, VA, USA).

Techniques: Over Expression, Expressing, Western Blot, Knockdown, Plasmid Preparation